Choline Acetyltransferase/ChAT Antibody (CL3173)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46620

Novus Biologicals, part of Bio-Techne
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NBP2-46620
NBP2-46620-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC, Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL3173

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Choline Acetyltransferase/ChAT Antibodies »

Product Specifications

Immunogen

Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence GLFSSYRLPGHTQDTLVAQNSSIMPEPEHVIVACCNQFFVLDVVINFRRLSEGDLFTQLRKIVKMASNEDERLPPIGLLTSDGRSEWAEARTVLVKDSTN

Epitope

GLFSSYRLPGHTQDT

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Choline Acetyltransferase/ChAT Antibody (CL3173)

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining in human placenta and liver tissues. Corresponding CHAT RNA-seq data are presented for the same tissues.
Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunocytochemistry/ Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]

Immunocytochemistry/Immunofluorescence: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620] - Staining of SK-MEL-30 cells using the Anti-CHAT monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody (CL3173) [NBP2-46620]
View 6 more images

Applications for Choline Acetyltransferase/ChAT Antibody (CL3173)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Choline Acetyltransferase/ChAT

Note: This is the same product as NB300-178. FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.

Alternate Names

CHAT, Choline acetylase

Entrez Gene IDs

1103 (Human)

Gene Symbol

CHAT

UniProt

P28329 (Human)

Additional Choline Acetyltransferase/ChAT Products

Product Documents for Choline Acetyltransferase/ChAT Antibody (CL3173)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Choline Acetyltransferase/ChAT Antibody (CL3173)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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