Choline Acetyltransferase/ChAT Antibody (CL3173) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43796

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL3173

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Choline Acetyltransferase/ChAT Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P28329, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Choline Acetyltransferase/ChAT Antibody (CL3173) - Azide and BSA Free

Choline Acetyltransferase/ChAT Antibody (CL3173) - Azide and BSA Free Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP3-43796]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP3-43796]

Analysis in human placenta and liver tissues using NBP3-43796 antibody. Corresponding Choline Acetyltransferase/ChAT RNA-seq data are presented for the same tissues.
Choline Acetyltransferase/ChAT Antibody (CL3173) - Azide and BSA Free Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP3-43796]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP3-43796]

Staining of human cerebral cortex shows moderate positivity in cholinergic neural fibers.
Choline Acetyltransferase/ChAT Antibody (CL3173) - Azide and BSA Free Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP3-43796]

Immunohistochemistry-Paraffin: Choline Acetyltransferase/ChAT Antibody [NBP3-43796]

Staining of human liver shows no positivity in hepatocytes as expected.
View 6 more images

Applications for Choline Acetyltransferase/ChAT Antibody (CL3173) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Choline Acetyltransferase/ChAT

Note: This is the same product as NB300-178. FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine. SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.

Alternate Names

CHAT, Choline acetylase

Gene Symbol

CHAT

Additional Choline Acetyltransferase/ChAT Products

Product Documents for Choline Acetyltransferase/ChAT Antibody (CL3173) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Choline Acetyltransferase/ChAT Antibody (CL3173) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.