Chromogranin A Antibody (PHE5) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34709
![Immunohistochemistry-Paraffin: Chromogranin A Antibody (PHE5) - Azide and BSA Free [NBP2-34709]](https://resources.bio-techne.com/images/products/Chromogranin-A-Antibody-PHE5-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-34709-img0002.jpg "Immunohistochemistry-Paraffin: Chromogranin A Antibody (PHE5) - Azide and BSA Free [NBP2-34709]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Monkey, Mouse (Negative), Rat (Negative)
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # PHE5
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Summary for Chromogranin A Antibody (PHE5) - Azide and BSA Free
Immunogen
Human pheochromocytoma (Uniprot: P10645)
Reactivity Notes
Does not react with Mouse or Rat.
Localization
Finely granular cytoplasmic
Specificity
Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.
Marker
Neuroendocrine Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-32958).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Chromogranin A Antibody (PHE5) - Azide and BSA Free
Immunohistochemistry-Paraffin: Chromogranin A Antibody (PHE5) - Azide and BSA Free [NBP2-34709]
Immunohistochemistry-Paraffin: Chromogranin A Antibody (PHE5) - Azide and BSA Free [NBP2-34709] - Formalin-fixed, paraffin-embedded human adrenal gland stained with Chromogranin A monoclonal antibody (PHE5).Applications for Chromogranin A Antibody (PHE5) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5-1ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Chromogranin A
Alternate Names
Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I
Gene Symbol
CHGA
Additional Chromogranin A Products
Product Documents for Chromogranin A Antibody (PHE5) - Azide and BSA Free
Product Specific Notices for Chromogranin A Antibody (PHE5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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