Chromogranin A Antibody (PHE5) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34709B

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NBP2-32958

Alexa Fluor 350

NBP2-34709AF350

Alexa Fluor 405

NBP2-34709AF405

Alexa Fluor 488

NBP2-34709AF488

Alexa Fluor 532

NBP2-34709AF532

Alexa Fluor 594

NBP2-34709AF594

Alexa Fluor 647

NBP2-34709AF647

Alexa Fluor 700

NBP2-34709AF700

Alexa Fluor 750

NBP2-34709AF750

Allophycocyanin

NBP2-34709APC

CoraFluor 1

NBP2-34709CL1

DyLight 350

NBP2-34709UV

DyLight 405

NBP2-34709V

DyLight 488

NBP2-34709G

DyLight 550

NBP2-34709R

DyLight 594

NBP2-34709DL594

DyLight 650

NBP2-34709C

DyLight 680

NBP2-34709FR

DyLight 755

NBP2-34709IR

FITC

NBP2-34709F

HRP

NBP2-34709H

Janelia Fluor 525

NBP2-34709JF525

Janelia Fluor 549

NBP2-34709JF549

Janelia Fluor 646

NBP2-34709JF646

Janelia Fluor 669

NBP2-34709JF669

mFluor Violet 450 SE

NBP2-34709MFV450

mFluor Violet 500 SE

NBP2-34709MFV500

mFluor Violet 610 SE

NBP2-34709MFV610

PE

NBP2-34709PE

PerCP

NBP2-34709PCP

Forumulation

Catalog #

Azide and BSA Free

NBP2-34709

IHC-Prediluted

NBP2-44769

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Monkey, Mouse (Negative), Rat (Negative)

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # PHE5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Chromogranin A Antibodies »

Product Specifications

Immunogen

Human pheochromocytoma (Uniprot: P10645)

Reactivity Notes

Does not react with Mouse or Rat.

Localization

Finely granular cytoplasmic

Specificity

Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheochromocytomas, paragangliomas, and other neuroendocrine tumors. Co-expression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms. Reportedly, co-expression of certain keratins and chromogranin indicates neuroendocrine lineage. The presence of strong anti-chromogranin staining and absence of anti-keratin staining should raise the possibility of paraganglioma. The co-expression of chromogranin and NSE is typical of neuroendocrine neoplasms. Most pituitary adenomas and prolactinomas readily express chromogranin.

Marker

Neuroendocrine Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Applications for Chromogranin A Antibody (PHE5) [Biotin]

Application

Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Chromogranin A

Chromogranin A (CgA) is an 86 kDa protein that is the major member of the granin family of acidic secretory glycoproteins located in neuroendocrine cells. CgA is believed to play a role in targeting peptide hormones and neurotransmitters to granules of the regulated pathways and inhibit hormone and neurotransmitter release. Also, the widespread distribution of CgA has made the measurement of circulating immunoreactive CgA a valuable tool in the diagnosis of neuroendocrine neoplasia, and CgA immunohistochemistry can help to identify the neuroendocrine nature of tumors. The N-terminal domain of CgA inhibits tumor necrosis factor a (TNFa) induced gap formation in human umbilical venous endothelial cells. CgA levels which reflect neuroendocrine differentiation of prostatic carcinoma may have a diagnostic, therapeutic and prognostic role in the management of prostate cancer patients.

Alternate Names

Betagranin, Catestatin, CHGA, Chromofungin, Pancreastatin, Parastatin, Parathyroid Secretory Protein 1, Pituitary Secretory Protein I, SP-I

Gene Symbol

CHGA

Additional Chromogranin A Products

Product Documents for Chromogranin A Antibody (PHE5) [Biotin]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Chromogranin A Antibody (PHE5) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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