Key Product Details

Species Reactivity

Validated:

Human, Bovine (Negative), Canine (Negative), Feline (Negative), Porcine (Negative)

Cited:

Human

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # Hs-3

Format

BSA Free

Concentration

1.0 mg/ml

View all Clusterin Antibodies »

Product Specifications

Immunogen

Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Specificity

Apolipoprotein J (Hs-3)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Clusterin/APOJ Antibody (Hs-3) - BSA Free

Western Blot: Clusterin/APOJ Antibody (Hs-3)BSA Free [NB500-552]

Western Blot: Clusterin/APOJ Antibody (Hs-3)BSA Free [NB500-552]

Western Blot: Clusterin/APOJ Antibody (Hs-3) [NB500-552] - Analysis of clusterin in human seminal plasma using anti-clusterin (Hs-3) purified.
Clusterin/APOJ Antibody (Hs-3) - BSA Free Western Blot: Clusterin/APOJ Antibody (Hs-3) - BSA Free [NB500-552]

Western Blot: Clusterin/APOJ Antibody (Hs-3) - BSA Free [NB500-552]

Analysis of human clusterin using mouse monoclonal antibody Hs-3 on lysates of MCF-7 cell line and MOLT-4 cell line (clusterin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-clusterin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Clusterin was detected around 40 kDa.

Applications for Clusterin/APOJ Antibody (Hs-3) - BSA Free

Application
Recommended Usage

ELISA

1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1 ug/ml
Application Notes
Use in Flow reported in scientific literature (PMID:33176829) Predicted molecular weight: 60 kDa.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Clusterin

Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP-40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid poly-peptide that is posttranslationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, alpha and beta, are associated through disulfide bonds. The beta subunit (also called ApoJalpha) corresponds to residues 23-227. The alpha subunit (also called ApoJbeta) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences beta-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer's disease pathogenesis.

Alternate Names

APOJ, CLI, CLU, SGP-2, SP-40, TRPM-2

Entrez Gene IDs

1191 (Human)

Gene Symbol

CLU

Additional Clusterin Products

Product Documents for Clusterin/APOJ Antibody (Hs-3) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Clusterin/APOJ Antibody (Hs-3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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