Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20109

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Mass Spectrometry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # SAIC-43B-8

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Clusterin Antibodies »

Product Specifications

Immunogen

Peptide "ASSIIDELFQDR" derived from Clusterin conjugated to KLH. Clusterin isoform 1 functions as extracellular chaperone that prevents aggregation of nonnative proteins

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Scientific Data Images for Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free

Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free

Western Blot: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] -

Western Blot: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - HeLa(A) (0.03ug/ml), MCF7(B) (0.01ug/ml) cell lysate and human brain cerebellum(C) (0.03ug/ml), human plasma (D) (0.03ug/ml) and human testes(E) (0.03ug/ml) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SAIC-43B-8 (NBP3-20109) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] -

Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of SAIC-43B-8 (NBP3-20109) (1:100 dilution) for 1h followed by Alexa Fluor(R) 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20109, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.
Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free

Flow Cytometry: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] -

Flow Cytometry: Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free [NBP3-20109] - Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (isotype control, black line) or the rabbit IgG version of SAIC-43B-8 (NBP3-20109, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor(R) 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Applications for Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Mass Spectrometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This Ab can be used in immunoMRM.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

0.02% Proclin 300

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.

Background: Clusterin

Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP-40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid poly-peptide that is posttranslationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, alpha and beta, are associated through disulfide bonds. The beta subunit (also called ApoJalpha) corresponds to residues 23-227. The alpha subunit (also called ApoJbeta) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences beta-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer's disease pathogenesis.

Alternate Names

APOJ, CLI, CLU, SGP-2, SP-40, TRPM-2

Gene Symbol

CLU

Additional Clusterin Products

Product Documents for Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Clusterin/APOJ Antibody (SAIC-43B-8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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