Collagen II Antibody (5B2.5) [DyLight 350]

Novus Biologicals, part of Bio-Techne | Catalog # NB600-844UV

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB600-844AF350

Alexa Fluor 405

NB600-844AF405

Alexa Fluor 488

NB600-844AF488

Alexa Fluor 532

NB600-844AF532

Alexa Fluor 594

NB600-844AF594

Alexa Fluor 647

NB600-844AF647

Alexa Fluor 700

NB600-844AF700

Alexa Fluor 750

NB600-844AF750

Allophycocyanin

NB600-844APC

Biotin

NB600-844B

CoraFluor 1

NB600-844CL1

DyLight 405

NB600-844V

DyLight 488

NB600-844G

DyLight 550

NB600-844R

DyLight 594

NB600-844DL594

DyLight 650

NB600-844C

DyLight 680

NB600-844FR

DyLight 755

NB600-844IR

FITC

NB600-844F

HRP

NB600-844H

Janelia Fluor 525

NB600-844JF525

Janelia Fluor 549

NB600-844JF549

Janelia Fluor 585

NB600-844JF585

Janelia Fluor 635

NB600-844JF635

Janelia Fluor 646

NB600-844JF646

Janelia Fluor 669

NB600-844JF669

mFluor Violet 450 SE

NB600-844MFV450

mFluor Violet 500 SE

NB600-844MFV500

mFluor Violet 610 SE

NB600-844MFV610

PE

NB600-844PE

PerCP

NB600-844PCP

Forumulation

Catalog #

BSA Free

NB600-844

Azide and BSA Free

NBP2-80676

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Chicken, Rabbit

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

DyLight 350 (Excitation = 353 nm, Emission = 432 nm)

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5B2.5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Collagen II Antibodies »

Product Summary for Collagen II Antibody (5B2.5) [DyLight 350]

Immunogen

A purified preparation of lathyritic type II collagen from embryonic chicken sternum

Reactivity Notes

Use in Rabbit reported in scientific literature (PMID:35441114).Rat reactivity reported in scientific literature (PMID: 24349545). Rabbit reactivity reported in scientific literature (PMID: 15621244). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information. Rabbit reactivity reported in scientific literature (PMID:33120203).

Localization

Cartilage matrix

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Applications for Collagen II Antibody (5B2.5) [DyLight 350]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Collagen II

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. In cartilage, Collagen Type II constitutes the bulk of the fibril. Sensitization with Collagen Type II induces an erosive polyarthritis in rats, mice and higher primates which can resemble rheumatoid arthritis and relapsing polychrondritis.

Alternate Names

Chondrocalcin, COL2A1, SEDC

Gene Symbol

COL2A1

Additional Collagen II Products

Product Documents for Collagen II Antibody (5B2.5) [DyLight 350]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Collagen II Antibody (5B2.5) [DyLight 350]

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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