Collagen II Antibody (5B2.5) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80676
![Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) - Azide and BSA Free [NBP2-80676]](https://resources.bio-techne.com/images/products/Collagen-II-Antibody-5B2-5-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-80676-img0001.jpg "Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) - Azide and BSA Free [NBP2-80676]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Chicken, Rabbit
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 5B2.5
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Summary for Collagen II Antibody (5B2.5) - Azide and BSA Free
Immunogen
A purified preparation of lathyritic type II collagen from embryonic chicken sternum
Reactivity Notes
Rat reactivity reported in scientific literature (PMID: 24349545). Rabbit reactivity reported in scientific literature (PMID: 15621244). Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Cartilage matrix
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for Collagen II Antibody (5B2.5) - Azide and BSA Free
Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) - Azide and BSA Free [NBP2-80676]
Immunohistochemistry-Paraffin: Collagen II Antibody (5B2.5) - Azide and BSA Free [NBP2-80676] - Analysis of a FFPE section of human cartilage using 1:200 dilution of Collagen II [5B2.5] antibody (NB600-844). The signal was developed using HRP-DAB method which followed counterstaining of the cells with hematoxylin. Image from the standard format of tApplications for Collagen II Antibody (5B2.5) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunohistochemistry
reported in scientific literature (PMID 22558278; 24242000)
Immunohistochemistry-Frozen
reported in scientific literature (PMID 22558278)
Western Blot
1-5ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Collagen II
Alternate Names
Chondrocalcin, COL2A1, SEDC
Gene Symbol
COL2A1
Additional Collagen II Products
Product Documents for Collagen II Antibody (5B2.5) - Azide and BSA Free
Product Specific Notices for Collagen II Antibody (5B2.5) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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