Collagen VII Antibody (LH7.2)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-2728
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # LH7.2
Concentration
1 mg/ml
Product Specifications
Immunogen
Cells from a single cell suspension of epidermal cells (obtained from fresh human neonatal foreskin) were lysed with Nonidet P40 in phosphate buffered saline and the insoluble pellet was sonicated to prepare insoluble fractions.
Epitope
The epitope is on the NC1 domain.
Localization
Secreted, extracellular space, extracellular matrix, basement membrane.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
250 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications for Collagen VII Antibody (LH7.2)
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Immunohistochemistry
1:10 - 1:500
Western Blot
1:100 - 1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Collagen VII
Alternate Names
Collagen 7, collagen alpha-1(VII) chain, collagen VII, alpha-1 polypeptide, collagen, type VII, alpha 1, Collagen-7, EBD1, EBDCT, EBR1, epidermolysis bullosa, dystrophic, dominant and recessive, LC collagen, long chain collagen, Long-chain collagen
Entrez Gene IDs
1294 (Human)
Gene Symbol
COL7A1
Additional Collagen VII Products
Product Documents for Collagen VII Antibody (LH7.2)
Product Specific Notices for Collagen VII Antibody (LH7.2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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