Collagen VII Antibody (LH7.2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08278

Novus Biologicals, part of Bio-Techne
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NBP3-08278
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Key Product Details

Species Reactivity

Human, Bovine, Equine, Goat, Guinea Pig, Sheep

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # LH7.2

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Collagen VII Antibodies »

Product Specifications

Immunogen

Recombinant full-length human Collagen VII protein (Uniprot: Q02388)

Localization

Secreted. Extracellular matrix. Basement Membrane.

Specificity

Recognizes an epitope located on collagenase digested Type VII collagen (150kDa), i.e. the non-helical carboxyl terminal region of the dimer. Binding is localized at the inferior border of the lamina densa as demonstrated by immunoelectron microscopy. Type VII is also found in the retina, where its function is unknown. It interacts with Laminin 5 and Fibronectin. Collagen VII is a stratified squamous epithelial basement membrane protein that forms anchoring fibrils which may contribute to epithelial basement membrane organization and adherence by interacting with extracellular matrix (ECM) proteins such as type IV collagen.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

290 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07751).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Collagen VII Antibody (LH7.2) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: Collagen VII Antibody (LH7.2) - Azide and BSA Free [NBP3-08278]

Immunocytochemistry/ Immunofluorescence: Collagen VII Antibody (LH7.2) - Azide and BSA Free [NBP3-08278]

Immunocytochemistry/Immunofluorescence: Collagen VII Antibody (LH7.2) - Azide and BSA Free [NBP3-08278] - Immunofluorescence Analysis of PFA-fixed HeLa cells labeling Collagen VIIusing Collagen VII Mouse Monoclonal Antibody (LH7.2) followed by goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot.
Immunohistochemistry-Paraffin: Collagen VII Antibody (LH7.2) - Azide and BSA Free [NBP3-08278]

Immunohistochemistry-Paraffin: Collagen VII Antibody (LH7.2) - Azide and BSA Free [NBP3-08278]

Immunohistochemistry-Paraffin: Collagen VII Antibody (LH7.2) - Azide and BSA Free [NBP3-08278] - Formalin-fixed, paraffin-embedded human Skin stained with Collagen VII Mouse Monoclonal Antibody (LH7.2).
Flow Cytometry: Collagen VII Antibody (LH7.2) - Azide and BSA Free [NBP3-08278]

Flow Cytometry: Collagen VII Antibody (LH7.2) - Azide and BSA Free [NBP3-08278]

Flow Cytometry: Collagen VII Antibody (LH7.2) - Azide and BSA Free [NBP3-08278] - Flow Cytometric Analysis of PFA-fixed HeLa cells using Collagen VII Mouse Monoclonal Antibody (LH7.2) followed by goat anti- Mouse IgG-CF488 (Blue); Isotype Control (Red)
View 1 more images

Applications for Collagen VII Antibody (LH7.2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Collagen VII

Type VII collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.

Alternate Names

Collagen 7, collagen alpha-1(VII) chain, collagen VII, alpha-1 polypeptide, collagen, type VII, alpha 1, Collagen-7, EBD1, EBDCT, EBR1, epidermolysis bullosa, dystrophic, dominant and recessive, LC collagen, long chain collagen, Long-chain collagen

Gene Symbol

COL7A1

Additional Collagen VII Products

Product Documents for Collagen VII Antibody (LH7.2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Collagen VII Antibody (LH7.2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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