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COX-2 Antibody (COX2/1941) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08272

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # COX2/1941

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary for COX-2 Antibody (COX2/1941) - Azide and BSA Free

Immunogen

Recombinant human COX-2 protein fragment (around aa 442-572) (exact sequence is proprietary) (Uniprot: P35354)

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07251).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for COX-2 Antibody (COX2/1941) - Azide and BSA Free

Western Blot: COX-2 Antibody (COX2/1941)Azide and BSA Free [NBP3-08272]

Western Blot: COX-2 Antibody (COX2/1941)Azide and BSA Free [NBP3-08272]

Western Blot: COX-2 Antibody (COX2/1941) - Azide and BSA Free [NBP3-08272] - Western Blot Analysis of human HepG2 cell lysate using COX-2 Mouse Monoclonal Antibody (COX2/1941).

Applications for COX-2 Antibody (COX2/1941) - Azide and BSA Free

Application
Recommended Usage

ELISA

2-4 ug/ml

Western Blot

1-2 ug/ml
Application Notes
ELISA: Use antibody at 2-4ug/ml. For coating, order antibody without BSA
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: COX-2

COX2 (Cyclooxygenase 2) is an inducible enzyme. It is involved in the response of cells to growth factors, tumor promoters, and cytokines that induce its expression. Given its role in synthesizing prostaglandins, COX2 is therefore of interest in studying immune response regulation. COX2 is induced by a wide variety of stimuli and was initially identified as immediate early growth response gene. In addition, COX2 expression markedly increased in 85-90% of human colorectal adenocarcinoma whereas COX1 levels remain unchanged.

Long Name

Cyclooxygenase 2

Alternate Names

COX2, PGHS-2, PHS-II, PTGS2

Gene Symbol

PTGS2

Additional COX-2 Products

Product Documents for COX-2 Antibody (COX2/1941) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for COX-2 Antibody (COX2/1941) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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