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CTGF/CCN2 Antibody (CL5339)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61415

Novus Biologicals, part of Bio-Techne
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NBP2-61415
NBP2-61415-25ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL5339

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for CTGF/CCN2 Antibody (CL5339)

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LPSPDCPFPRRVKLPGKCCEEWVCDEPKDQTVVGPALAAYRLEDTFGPDPTMIRANCLVQTTEWSACSKTCGMG

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for CTGF/CCN2 Antibody (CL5339)

Western Blot: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]

Western Blot: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]

Western Blot: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Analysis in human cell line U-251 MG.
Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]

Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]

Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of rat cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.
Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]

Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415]

Immunohistochemistry-Paraffin: CTGF/CCN2 Antibody (CL5339) [NBP2-61415] - Staining of human cerebral cortex shows strong cytoplasmic positivity in layer 6b neurons.

Applications for CTGF/CCN2 Antibody (CL5339)

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CTGF/CCN2

Connective Tissue Growth Factor (CTGF) is a 38kDa, cysteine-rich, secreted peptide. CTGF promotes endothelial cell growth, migration, adhesion and survival. and is thus implicated in endothelial cell function and angiogenesis. CTGF is implicated in are embryogenesis, wound healing and regulation of extracellular matrix production, and is required for normal skeletal growth.

CTGF antibodies are useful tools for angiogenesis and cell structure research, and for studies on certian types of cancer.

Long Name

Connective Tissue Growth Factor

Alternate Names

CCN2, CTGRP, Fisp12, HCS24, IGFBP-8, NOV2

Gene Symbol

CCN2

Additional CTGF/CCN2 Products

Product Documents for CTGF/CCN2 Antibody (CL5339)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for CTGF/CCN2 Antibody (CL5339)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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