Cytochrome P450 1A2 Antibody (OTI8F1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02563

Novus Biologicals, part of Bio-Techne
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NBP2-02563
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI8F1

Concentration

0.77 mg/ml

View all Cytochrome P450 1A2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CYP1A2 (NP_000752) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

58.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytochrome P450 1A2 Antibody (OTI8F1)

Western Blot: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563]

Western Blot: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563]

Western Blot: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 1A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytochrome P450 1A2.
Immunocytochemistry/ Immunofluorescence: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563]

Immunocytochemistry/ Immunofluorescence: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563]

Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP1A2 .
Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563]

Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563]

Immunohistochemistry-Paraffin: Cytochrome P450 1A2 Antibody (OTI8F1) [NBP2-02563] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.
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Applications for Cytochrome P450 1A2 Antibody (OTI8F1)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.77 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytochrome P450 1A2

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region.

Long Name

Cytochrome P450 1A2

Alternate Names

CYP1A2, CYPIA2, Cytochrome P(3)450, Cytochrome P450 4, Cytochrome P450-P3

Entrez Gene IDs

1544 (Human)

Gene Symbol

CYP1A2

Additional Cytochrome P450 1A2 Products

Product Documents for Cytochrome P450 1A2 Antibody (OTI8F1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytochrome P450 1A2 Antibody (OTI8F1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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