Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70518

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI8F1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Cytochrome P450 1A2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human CYP1A2 (NP_000752) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

58.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free

Western Blot: Cytochrome P450 1A2 Antibody (OTI8F1)Azide and BSA Free [NBP2-70518]

Western Blot: Cytochrome P450 1A2 Antibody (OTI8F1)Azide and BSA Free [NBP2-70518]

Western Blot: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytochrome P450 1A2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cy
Immunocytochemistry/ Immunofluorescence: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518]

Immunocytochemistry/ Immunofluorescence: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518]

Immunocytochemistry/Immunofluorescence: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY CYP1A2 .
Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518]

Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518]

Immunohistochemistry: Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free [NBP2-70518] - Staining of paraffin-embedded Human prostate tissue using anti-Cytochrome P450 1A2 mouse monoclonal antibody.
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Applications for Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytochrome P450 1A2

In animals, P450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs. The four major families involved in drug metabolism are CYP 1,2,3, and 4. CYP1A2 is an aryl hydrocarbon hydroxylase involved in the metabolism of endogenous compounds and xenobiotics. It is one of the major drug metabolising enzymes in humans.

Long Name

Cytochrome P450 1A2

Alternate Names

CYP1A2, CYPIA2, Cytochrome P(3)450, Cytochrome P450 4, Cytochrome P450-P3

Gene Symbol

CYP1A2

Additional Cytochrome P450 1A2 Products

Product Documents for Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytochrome P450 1A2 Antibody (OTI8F1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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