Cytochrome P450 2E1 Antibody (M12P4H2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07825
![Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (M12P4H2) [NBP3-07825]](https://resources.bio-techne.com/images/products/Cytochrome-P450-2E1-Antibody-M12P4H2-Immunohistochemistry-Paraffin-NBP3-07825-img0001.jpg "Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (M12P4H2) [NBP3-07825]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # M12P4H2
Concentration
0.2 mg/ml
Product Summary for Cytochrome P450 2E1 Antibody (M12P4H2)
Immunogen
Ovalbumin-conjugated synthetic peptide HIGFGCIPPR (C-terminal sequence) (Uniprot: P05181)
Localization
Endoplasmic reticulum membrane, Microsome membrane
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1mg/ml. (NBP3-08831)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Cytochrome P450 2E1 Antibody (M12P4H2)
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (M12P4H2) [NBP3-07825]
Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (M12P4H2) [NBP3-07825] - Formalin-fixed, paraffin-embedded human Hepatic Carcinoma stained with Cytochrome P450 2E1 Mouse Monoclonal Antibody (M12P4H2).Applications for Cytochrome P450 2E1 Antibody (M12P4H2)
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.1-0.2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytochrome P450 2E1
Alternate Names
4-nitrophenol 2-hydroxylase, CPE1, CYP2Ecytochrome P450 2E1, CYPIIE1, cytochrome P450, family 2, subfamily E, polypeptide 1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, Cytochrome P450-J, EC 1.14.13.-, EC 1.14.13.n7, EC 1.14.14.1, flavoprotein-linked monooxygenase, microsomal monooxygenase, P450C2E, P450-J, xenobiotic monooxygenase
Gene Symbol
CYP2E1
UniProt
Additional Cytochrome P450 2E1 Products
Product Documents for Cytochrome P450 2E1 Antibody (M12P4H2)
Product Specific Notices for Cytochrome P450 2E1 Antibody (M12P4H2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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