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Cytochrome P450 2E1 Antibody (M12P4H2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08831

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # M12P4H2

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Summary for Cytochrome P450 2E1 Antibody (M12P4H2) - Azide and BSA Free

Immunogen

Ovalbumin-conjugated synthetic peptide HIGFGCIPPR (C-terminal sequence) (Uniprot: P05181)

Localization

Endoplasmic reticulum membrane, Microsome membrane

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07825).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for Cytochrome P450 2E1 Antibody (M12P4H2) - Azide and BSA Free

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (M12P4H2) - Azide and BSA Free [NBP3-08831]

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (M12P4H2) - Azide and BSA Free [NBP3-08831]

Immunohistochemistry-Paraffin: Cytochrome P450 2E1 Antibody (M12P4H2) - Azide and BSA Free [NBP3-08831] - Formalin-fixed, paraffin-embedded human Hepatic Carcinoma stained with Cytochrome P450 2E1 Mouse Monoclonal Antibody (M12P4H2).

Applications for Cytochrome P450 2E1 Antibody (M12P4H2) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

0.1-0.2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 0.1-0.2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Cytochrome P450 2E1

Alternate Names

4-nitrophenol 2-hydroxylase, CPE1, CYP2Ecytochrome P450 2E1, CYPIIE1, cytochrome P450, family 2, subfamily E, polypeptide 1, cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1, Cytochrome P450-J, EC 1.14.13.-, EC 1.14.13.n7, EC 1.14.14.1, flavoprotein-linked monooxygenase, microsomal monooxygenase, P450C2E, P450-J, xenobiotic monooxygenase

Gene Symbol

CYP2E1

Additional Cytochrome P450 2E1 Products

Product Documents for Cytochrome P450 2E1 Antibody (M12P4H2) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytochrome P450 2E1 Antibody (M12P4H2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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