Cytokeratin 10/13 Antibody (DE-K13)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-45256

Novus Biologicals, part of Bio-Techne
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NBP2-45256-0.02mg
NBP2-45256-0.1mg
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Key Product Details

Species Reactivity

Human, Feline

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # DE-K13

Concentration

0.2 mg/ml

View all Cytokeratin 10/13 Antibodies »

Product Specifications

Immunogen

Cytoskeletal preparation extracted from human ectocervical epithelium

Localization

Cytoplasmic

Specificity

This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this monoclonal antibody serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11497)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Cytokeratin 10/13 Antibody (DE-K13)

Immunocytochemistry/ Immunofluorescence: Cytokeratin 10/13 Antibody (DE-K13) [NBP2-45256]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 10/13 Antibody (DE-K13) [NBP2-45256]

Immunocytochemistry/Immunofluorescence: Cytokeratin 10/13 Antibody (DE-K13) [NBP2-45256] - Immunofluorescent analysis of MeOH-fixed MCF-7 cells using Cytokeratin 10/13Mouse Monoclonal Antibody (DE-K13) followed by goat anti-mouse IgG-CF488. Nuclei counterstained with RedDot.
Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (DE-K13) [NBP2-45256]

Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (DE-K13) [NBP2-45256]

Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (DE-K13) [NBP2-45256] - Human Tonsil stained with Cytokeratin 10/13 Monoclonal Antibody (DE-K13)

Applications for Cytokeratin 10/13 Antibody (DE-K13)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

0.5 - 1.0 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 10/13

Alternate Names

BCIE, BIE, CK10, CK-10, cytokeratin 10, Cytokeratin-10, EHK, K10keratosis palmaris et plantaris, keratin 10, keratin, type I cytoskeletal 10, Keratin-10, KPP

Gene Symbol

KRT10

UniProt

P13646 (Human), P13645 (Human)

Additional Cytokeratin 10/13 Products

Product Documents for Cytokeratin 10/13 Antibody (DE-K13)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 10/13 Antibody (DE-K13)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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