Cytokeratin 10/13 Antibody (DE-K13) - IHC-Prediluted

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48185

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Feline

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # DE-K13

Format

IHC-Prediluted

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Cytokeratin 10/13 Antibodies »

Product Specifications

Immunogen

Cytoskeletal preparation extracted from human ectocervical epithelium

Localization

Cytoplasmic

Specificity

This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this monoclonal antibody serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Scientific Data Images for Cytokeratin 10/13 Antibody (DE-K13) - IHC-Prediluted

Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (DE-K13) - IHC-Prediluted [NBP2-48185]

Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (DE-K13) - IHC-Prediluted [NBP2-48185]

Immunohistochemistry-Paraffin: Cytokeratin 10/13 Antibody (DE-K13) - IHC-Prediluted [NBP2-48185] - Formalin-fixed, paraffin-embedded human Tonsil stained with Cytokeratin 10/13 Monoclonal Antibody (DE-K13)

Applications for Cytokeratin 10/13 Antibody (DE-K13) - IHC-Prediluted

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Hu-chromosome location: 17q21.2
Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Format

IHC-Prediluted

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin 10/13

Alternate Names

BCIE, BIE, CK10, CK-10, cytokeratin 10, Cytokeratin-10, EHK, K10keratosis palmaris et plantaris, keratin 10, keratin, type I cytoskeletal 10, Keratin-10, KPP

Gene Symbol

KRT10

Additional Cytokeratin 10/13 Products

Product Documents for Cytokeratin 10/13 Antibody (DE-K13) - IHC-Prediluted

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Cytokeratin 10/13 Antibody (DE-K13) - IHC-Prediluted

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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