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Cytokeratin 10/13 Antibody (DE-K13) [DyLight 488]

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11497G

Novus Biologicals, part of Bio-Techne
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NBP3-11497G

Key Product Details

Species Reactivity

Validated:

Human, Feline

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

DyLight 488 (Excitation = 493 nm, Emission = 518 nm)

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # DE-K13

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Cytokeratin 10/13 Antibody (DE-K13) [DyLight 488]

Immunogen

Cytoskeletal preparation extracted from human ectocervical epithelium

Localization

Cytoplasmic

Specificity

This antibody recognizes cytokeratin 10 (56.5kDa) and cytokeratin 13 (53kDa) in Western blotting. It recognizes only cytokeratin 13 in formalin-fixed, paraffin-embedded tissue sections. It does not react with cytokeratin 10 positive, cytokeratin 13 negative epithelia such as epidermis. However, on frozen sections this monoclonal antibody serves as differentiation-related marker of all stratified epithelia; it stains all suprabasal cells in both cornifying and non-cornifying stratified epithelia and more differentiated cells of squamous carcinomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Applications for Cytokeratin 10/13 Antibody (DE-K13) [DyLight 488]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Cytokeratin 10/13

Alternate Names

BCIE, BIE, CK10, CK-10, cytokeratin 10, Cytokeratin-10, EHK, K10keratosis palmaris et plantaris, keratin 10, keratin, type I cytoskeletal 10, Keratin-10, KPP

Gene Symbol

KRT10

Additional Cytokeratin 10/13 Products

Product Documents for Cytokeratin 10/13 Antibody (DE-K13) [DyLight 488]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytokeratin 10/13 Antibody (DE-K13) [DyLight 488]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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