Cytokeratin 19 Antibody (OTI3F8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48277

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Rat, Canine, Monkey, Primate

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3F8

Concentration

0.15 mg/ml

View all Cytokeratin 19 Antibodies »

Product Summary for Cytokeratin 19 Antibody (OTI3F8)

Immunogen

Recombinant fragment expressed in E.coli corresponding to amino acids 240-390 of human CK19

Specificity

This antibody is specific for Homo sapiens keratin 19 (KRT19).

Marker

Epithelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 19 Antibody (OTI3F8)

Western Blot: Cytokeratin 19 Antibody (3F8) [NBP1-48277] Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytokeratin 19 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (OTI3F8) [NBP1-48277]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (OTI3F8) [NBP1-48277]

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (3F8) [NBP1-48277] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytokeratin 19.
Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (OTI3F8) [NBP1-48277]

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (OTI3F8) [NBP1-48277]

Immunohistochemistry-Paraffin: Cytokeratin 19 Antibody (3F8) [NBP1-48277] - Staining of paraffin-embedded liver tissue using anti-Cytokeratin 19mouse monoclonal antibody.
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Applications for Cytokeratin 19 Antibody (OTI3F8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.15 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 19

Keratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis.

Alternate Names

CK19, EndoC, K19, Krt19

Entrez Gene IDs

3880 (Human)

Gene Symbol

KRT19

UniProt

P08727

Additional Cytokeratin 19 Products

Product Documents for Cytokeratin 19 Antibody (OTI3F8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 19 Antibody (OTI3F8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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