Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71092
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Rat, Canine, Monkey, Primate
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI3F8
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Recombinant fragment expressed in E.coli corresponding to amino acids 240-390 of human CK19
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
43.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free
Western Blot: Cytokeratin 19 Antibody (OTI3F8)Azide and BSA Free [NBP2-71092]
Western Blot: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 19 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CytokerImmunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092]
Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytokeratin 19.Immunohistochemistry: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092]
Immunohistochemistry: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092] - Staining of paraffin-embedded Kidney tissue using anti-Cytokeratin 19mouse monoclonal antibody.Applications for Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:50 - 1:150
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Cytokeratin 19
Alternate Names
CK19, EndoC, K19, Krt19
Gene Symbol
KRT19
Additional Cytokeratin 19 Products
Product Documents for Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free
Product Specific Notices for Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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