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Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71092

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Rat, Canine, Monkey, Primate

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3F8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Recombinant fragment expressed in E.coli corresponding to amino acids 240-390 of human CK19

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

43.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free

Western Blot: Cytokeratin 19 Antibody (OTI3F8)Azide and BSA Free [NBP2-71092]

Western Blot: Cytokeratin 19 Antibody (OTI3F8)Azide and BSA Free [NBP2-71092]

Western Blot: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 19 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytoker
Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092]

Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytokeratin 19.
Immunohistochemistry: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092]

Immunohistochemistry: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092]

Immunohistochemistry: Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free [NBP2-71092] - Staining of paraffin-embedded Kidney tissue using anti-Cytokeratin 19mouse monoclonal antibody.

Applications for Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50 - 1:150

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 19

Cytokeratin 19 (also known as Keratin, type I cytoskeletal 19) belongs to the keratin family which consists of fibrous proteins that make up the framework of epithelial cells. Cytokeratin 19 plays a role in the organization of myofibers. This protein is known to have interactions with ZNF638, FANCG, FAM107A, KRT15 and EXOC8. Cytokeratin 19 has been studied in relation to several disorders and diseases including chordoma, adenoma, hamartoma, adenoiditis, carcinoma, and liver disease.

Alternate Names

CK19, EndoC, K19, Krt19

Gene Symbol

KRT19

Additional Cytokeratin 19 Products

Product Documents for Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytokeratin 19 Antibody (OTI3F8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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