Cytokeratin 5/8 Antibody (SPM268)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44703
![Immunohistochemistry-Paraffin: Cytokeratin 5/8 Antibody (SPM268) [NBP2-44703]](https://resources.bio-techne.com/images/products/Cytokeratin-5-8-Antibody-SPM268-Immunohistochemistry-Paraffin-NBP2-44703-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5/8 Antibody (SPM268) [NBP2-44703]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken (Negative), Ferret, Hamster, Sheep, Xenopus (Negative)
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM268
Concentration
0.2 mg/ml
Product Summary for Cytokeratin 5/8 Antibody (SPM268)
Immunogen
Cytoskeletal preparation from HeLa cells
Reactivity Notes
Does not react with Chicken or Xenopus.
Localization
Cytoplasmic
Specificity
It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
0.5 ml at 200 ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G chromatography. Supplied in 10 mM PBS, pH 7.4 with 0.05% BSA and 0.05% sodium azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47823)
Store at 2 to 8C.
Store at 2 to 8C.
Scientific Data Images for Cytokeratin 5/8 Antibody (SPM268)
Immunohistochemistry-Paraffin: Cytokeratin 5/8 Antibody (SPM268) [NBP2-44703]
Immunohistochemistry-Paraffin: Cytokeratin 5/8 Antibody (SPM268) [NBP2-44703] - Human Colon Carcinoma stained with Cytokeratin 5/8 Monoclonal Antibody (SPM268).Applications for Cytokeratin 5/8 Antibody (SPM268)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS, pH 7.4 with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 5/8
Alternate Names
cell proliferation-inducing protein 46, CK18, CK5, CYK18, DDD, K18, K1CO, K5, Keratin, Keratin 18, Keratin 5, KRT18, krt5, KRT5A, KRTB, type I cytoskeletal 18, type II cytoskeletal 5
Gene Symbol
KRT5
UniProt
Additional Cytokeratin 5/8 Products
Product Documents for Cytokeratin 5/8 Antibody (SPM268)
Product Specific Notices for Cytokeratin 5/8 Antibody (SPM268)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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