Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-48114
![Immunohistochemistry-Paraffin: Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted [NBP2-48114]](https://resources.bio-techne.com/images/products/Cytokeratin-5-8-Antibody-SPM268-IHC-Prediluted-Immunohistochemistry-Paraffin-NBP2-48114-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted [NBP2-48114]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken (Negative), Ferret, Hamster, Sheep, Xenopus (Negative)
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM268
Format
IHC-Prediluted
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted
Immunogen
Cytoskeletal preparation from HeLa cells
Reactivity Notes
Does not react with Chicken or Xenopus.
Localization
Cytoplasmic
Specificity
It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.
Scientific Data Images for Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted
Immunohistochemistry-Paraffin: Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted [NBP2-48114]
Immunohistochemistry-Paraffin: Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted [NBP2-48114] - Human Colon Carcinoma stained with Cytokeratin 5/8 Monoclonal Antibody (SPM268).Applications for Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted
Application
Recommended Usage
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS, pH 7.4 with 0.05% BSA
Format
IHC-Prediluted
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Cytokeratin 5/8
Alternate Names
cell proliferation-inducing protein 46, CK18, CK5, CYK18, DDD, K18, K1CO, K5, Keratin, Keratin 18, Keratin 5, KRT18, krt5, KRT5A, KRTB, type I cytoskeletal 18, type II cytoskeletal 5
Gene Symbol
KRT5
Additional Cytokeratin 5/8 Products
Product Documents for Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted
Product Specific Notices for Cytokeratin 5/8 Antibody (SPM268) - IHC-Prediluted
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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