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Cytokeratin 5/8 Antibody (SPM268) [Janelia Fluor® 669]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47823JF669

Novus Biologicals, part of Bio-Techne
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NBP2-47823JF669

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken (Negative), Ferret, Hamster, Sheep, Xenopus (Negative)

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Janelia Fluor 669

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM268

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Cytokeratin 5/8 Antibody (SPM268) [Janelia Fluor® 669]

Immunogen

Cytoskeletal preparation from HeLa cells

Reactivity Notes

Does not react with Chicken or Xenopus.

Localization

Cytoplasmic

Specificity

It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Applications for Cytokeratin 5/8 Antibody (SPM268) [Janelia Fluor® 669]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Cytokeratin 5/8

Alternate Names

cell proliferation-inducing protein 46, CK18, CK5, CYK18, DDD, K18, K1CO, K5, Keratin, Keratin 18, Keratin 5, KRT18, krt5, KRT5A, KRTB, type I cytoskeletal 18, type II cytoskeletal 5

Gene Symbol

KRT5

Additional Cytokeratin 5/8 Products

Product Documents for Cytokeratin 5/8 Antibody (SPM268) [Janelia Fluor® 669]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cytokeratin 5/8 Antibody (SPM268) [Janelia Fluor® 669]



Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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