Cytokeratin 8 Antibody (OTI1B12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-48281

Novus Biologicals, part of Bio-Techne
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NBP1-48281
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1B12

Concentration

1 mg/ml

View all Cytokeratin 8 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens keratin 8 (KRT8).

Marker

Epithelial Cell Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

53.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 8 Antibody (OTI1B12)

Western Blot: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Western Blot: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Western Blot: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Analysis of extracts (35ug) from 9 different cell lines by using anti-Cytokeratin 8 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytokeratin 8.
Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281]

Immunohistochemistry-Paraffin: Cytokeratin 8 Antibody (OTI1B12) [NBP1-48281] - Cytokeratin 8 antibody staining of mouse colon epithelium. IHC-P image submitted by a verified customer review.
View 9 more images

Applications for Cytokeratin 8 Antibody (OTI1B12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2500-5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using NBP1-48281 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 8

Keratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

Alternate Names

CARD2, CK8, EndoA, KRT8

Entrez Gene IDs

3856 (Human)

Gene Symbol

KRT8

Additional Cytokeratin 8 Products

Product Documents for Cytokeratin 8 Antibody (OTI1B12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 8 Antibody (OTI1B12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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