Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71082

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1B12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Cytokeratin 8 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 91-381 of human CK8 (NP_002264) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

53.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free

Western Blot: Cytokeratin 8 Antibody (OTI1B12)Azide and BSA Free [NBP2-71082]

Western Blot: Cytokeratin 8 Antibody (OTI1B12)Azide and BSA Free [NBP2-71082]

Western Blot: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cytokeratin 8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Cytokera
Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082]

Immunocytochemistry/ Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082]

Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cytokeratin 8.
Immunohistochemistry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082]

Immunohistochemistry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082]

Immunohistochemistry: Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free [NBP2-71082] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KRT8 mouse monoclonal antibody at 1:150 dilution.
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Applications for Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Immunohistochemistry

1:50 - 1:150

Western Blot

1:2500-5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cytokeratin 8

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Alternate Names

CARD2, CK8, EndoA, KRT8

Gene Symbol

KRT8

Additional Cytokeratin 8 Products

Product Documents for Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin 8 Antibody (OTI1B12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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