Cytokeratin, HMW Antibody (rKRTH/6617)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07645

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rKRTH/6617

Concentration

0.2 mg/ml

View all Cytokeratin, HMW Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to Cytokeratin, HMW residues within aa300-400 of Cytokeratin, HMW (Uniprot: Q01546)

Localization

Cytoplasmic

Specificity

The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type I cytokeratins antibody.

Marker

Epithelial Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08675)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Cytokeratin, HMW Antibody (rKRTH/6617)

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (rKRTH/6617) [NBP3-07645]

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (rKRTH/6617) [NBP3-07645]

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (rKRTH/6617) [NBP3-07645] - Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin, HMW Recombinant Mouse Monoclonal Antibody (rKRTH/6617).
Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (rKRTH/6617) [NBP3-07645]

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (rKRTH/6617) [NBP3-07645]

Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (rKRTH/6617) [NBP3-07645] - Formalin-fixed, paraffin-embedded human sebaceous gland stained with Cytokeratin, HMW Recombinant Mouse Monoclonal Antibody (rKRTH/6617).

Applications for Cytokeratin, HMW Antibody (rKRTH/6617)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Cytokeratin, HMW

KRT76, also known as Keratin, type II cytoskeletal 2 oral, is a 638 amino acid that is approx. 66 kDa, belongs to the intermediate filament family responsible for the structural integrity of epithelial cells, subdivided into epithelial keratins and hair keratins, and probably contributes to terminal cornification. Studies are being performed in relation to this protein and obstructive jaundice, pharyngitis, jaundice, cervical cancer, neuronitis, colorectal cancer, cervicitis, interferon and hepatitis. This protein has been shown to have interactions with ATG13, PDPK1, ULK2, GABARAP, and PRKAA1 in cytoskeleton organization pathways.

Alternate Names

CK-2P, cytokeratin 2, Cytokeratin-2P, HUMCYT2A, K2P, K76, keratin 76, keratin, type II cytoskeletal 2 oral, keratin-76, KRT2Bcytokeratin-2P, KRT2Pkeratin 2p, KRT76, Type-II keratin Kb9

Gene Symbol

KRT76

UniProt

Q01546 (Human)

Additional Cytokeratin, HMW Products

Product Documents for Cytokeratin, HMW Antibody (rKRTH/6617)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Cytokeratin, HMW Antibody (rKRTH/6617)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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