Cytokeratin, HMW Antibody (rKRTH/6617) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08675
Recombinant Monoclonal Antibody
![Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (rKRTH/6617) - Azide and BSA Free [NBP3-08675]](https://resources.bio-techne.com/images/products/Cytokeratin-HMW-Antibody-rKRTH-6617-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08675-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (rKRTH/6617) - Azide and BSA Free [NBP3-08675]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # rKRTH/6617
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide corresponding to Cytokeratin, HMW residues within aa300-400 of Cytokeratin, HMW (Uniprot: Q01546)
Localization
Cytoplasmic
Specificity
The keratins are the typical intermediate filament proteins of epithelia, showing an outstanding degree of molecular diversity. Heteropolymeric filaments are formed by pairing of type I and type II molecules. In humans 54 functional keratin genes exist. They are expressed in highly specific patterns related to the epithelial type and stage of cellular differentiation. This antibody can detect high molecular weight CK1, CK2, CK3, CK4, CK5, CK6, CK7 and CK8. It is usually used in a cytokeratin cocktail with type I cytokeratins antibody.
Marker
Epithelial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07645).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for Cytokeratin, HMW Antibody (rKRTH/6617) - Azide and BSA Free
Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (rKRTH/6617) - Azide and BSA Free [NBP3-08675]
Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (rKRTH/6617) - Azide and BSA Free [NBP3-08675] - Formalin-fixed, paraffin-embedded human sebaceous gland stained with Cytokeratin, HMW Recombinant Mouse Monoclonal Antibody (rKRTH/6617).![Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (rKRTH/6617) - Azide and BSA Free [NBP3-08675]](https://resources.bio-techne.com/images/products/Cytokeratin-HMW-Antibody-rKRTH-6617-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP3-08675-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (rKRTH/6617) - Azide and BSA Free [NBP3-08675]")
Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (rKRTH/6617) - Azide and BSA Free [NBP3-08675]
Immunohistochemistry-Paraffin: Cytokeratin, HMW Antibody (rKRTH/6617) - Azide and BSA Free [NBP3-08675] - Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin, HMW Recombinant Mouse Monoclonal Antibody (rKRTH/6617).Applications for Cytokeratin, HMW Antibody (rKRTH/6617) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Cytokeratin, HMW
Alternate Names
CK-2P, cytokeratin 2, Cytokeratin-2P, HUMCYT2A, K2P, K76, keratin 76, keratin, type II cytoskeletal 2 oral, keratin-76, KRT2Bcytokeratin-2P, KRT2Pkeratin 2p, KRT76, Type-II keratin Kb9
Gene Symbol
KRT76
Additional Cytokeratin, HMW Products
Product Documents for Cytokeratin, HMW Antibody (rKRTH/6617) - Azide and BSA Free
Product Specific Notices for Cytokeratin, HMW Antibody (rKRTH/6617) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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