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DAP Kinase 2 Antibody (OTI1C5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02477

Novus Biologicals, part of Bio-Techne
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NBP2-02477
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # OTI1C5

Concentration

0.76 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human DAPK2 (NP_055141) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Theoretical MW

42.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DAP Kinase 2 Antibody (OTI1C5)

Western Blot: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Western Blot: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Western Blot: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Analysis of extracts (35ug) from 9 different cell lines by usin g anti-DAP Kinase 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunocytochemistry/ Immunofluorescence: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunocytochemistry/Immunofluorescence: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DAP Kinase 2.
Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477]

Immunohistochemistry-Paraffin: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of paraffin-embedded Human pancreas tissue using anti-DAP Kinase 2 mouse monoclonal antibody.

Applications for DAP Kinase 2 Antibody (OTI1C5)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:1000-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.76 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DAPK2

This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. [provided by RefSeq].

Long Name

Death-associated Protein Kinase 2

Alternate Names

DAP Kinase 2, DRP-1

Entrez Gene IDs

23604 (Human)

Gene Symbol

DAPK2

Additional DAPK2 Products

Product Documents for DAP Kinase 2 Antibody (OTI1C5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DAP Kinase 2 Antibody (OTI1C5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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