DAP Kinase 2 Antibody (OTI1C5) [Allophycocyanin/Cy7]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71753APCCY7

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Unconjugated

NBP2-02477

Alexa Fluor 350

NBP2-71753AF350

Alexa Fluor 405

NBP2-71753AF405

Alexa Fluor 488

NBP2-71753AF488

Alexa Fluor 532

NBP2-71753AF532

Alexa Fluor 594

NBP2-71753AF594

Alexa Fluor 647

NBP2-71753AF647

Alexa Fluor 700

NBP2-71753AF700

Alexa Fluor 750

NBP2-71753AF750

Allophycocyanin

NBP2-71753APC

Biotin

NBP2-71753B

CoraFluor 1

NBP2-71753CL1

DyLight 350

NBP2-71753UV

DyLight 405

NBP2-71753V

DyLight 488

NBP2-71753G

DyLight 550

NBP2-71753R

DyLight 594

NBP2-71753DL594

DyLight 650

NBP2-71753C

DyLight 680

NBP2-71753FR

DyLight 755

NBP2-71753IR

FITC

NBP2-71753F

HRP

NBP2-71753H

Janelia Fluor 525

NBP2-71753JF525

Janelia Fluor 549

NBP2-71753JF549

Janelia Fluor 585

NBP2-71753JF585

Janelia Fluor 635

NBP2-71753JF635

Janelia Fluor 646

NBP2-71753JF646

Janelia Fluor 669

NBP2-71753JF669

mFluor Violet 450 SE

NBP2-71753MFV450

mFluor Violet 500 SE

NBP2-71753MFV500

mFluor Violet 610 SE

NBP2-71753MFV610

PE

NBP2-71753PE

PE/Atto594

NBP2-71753PEATT594

PE/Cy5.5

NBP2-71753PECY55

PE/Cy7

NBP2-71753PECY7

PerCP

NBP2-71753PCP

Forumulation

Catalog #

Azide and BSA Free

NBP2-71753

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Allophycocyanin/Cy7 (Excitation = 650;755 nm, Emission = 767 nm)

Antibody Source

Monoclonal Mouse IgG₃ Clone # OTI1C5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all DAPK2 Antibodies »

Product Specifications

Immunogen

Full length recombinant protein of human DAP Kinase 2 (NP_055141) produced in HEK293T cells.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₃

Applications for DAP Kinase 2 Antibody (OTI1C5) [Allophycocyanin/Cy7]

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: DAPK2

Protein kinase termed death-associated protein kinase 2 (DAPK2) dependant on calcium/calmodulin (Ca2+/CaM) contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a protein kinase involved in apoptosis. Overexpression of DAPK2 significantly induced the morphological changes characteristic of apoptosis. Results indicate that DAPK2 is an additional member of DAP kinase family involved in apoptotic signaling (1). The region of homology spans the catalytic domain and the CaM-regulatory region, whereas the remaining C-terminal part of the protein differs completely from DAP kinase and displays no homology to any known protein. The catalytic domain is also homologous to the recently identified ZIP kinase and to a lesser extent to the catalytic domains of DRAK1 and -2 (2). DAPK2 has a novel regulatory mechanism that controls its pro-apoptotic functions. It comprises a single autophosphorylation event mapped to Ser308 within the CaM regulatory domain. A negative charge at this site reduces both the binding to CaM and the formation of DRP-1 homodimers (3).

Long Name

Death-associated Protein Kinase 2

Alternate Names

DAP Kinase 2, DRP-1

Gene Symbol

DAPK2

Additional DAPK2 Products

Product Documents for DAP Kinase 2 Antibody (OTI1C5) [Allophycocyanin/Cy7]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for DAP Kinase 2 Antibody (OTI1C5) [Allophycocyanin/Cy7]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews

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Flow Cytometry