DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-71753

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # OTI1C5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all DAPK2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DAPK2 (NP_055141) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Theoretical MW

42.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free

Western Blot: DAP Kinase 2 Antibody (OTI1C5)Azide and BSA Free [NBP2-71753]

Western Blot: DAP Kinase 2 Antibody (OTI1C5)Azide and BSA Free [NBP2-71753]

Western Blot: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP Kinase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAP Kinas
Immunocytochemistry/ Immunofluorescence: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753]

Immunocytochemistry/ Immunofluorescence: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753]

Immunocytochemistry/Immunofluorescence: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DAP Kinase 2.
Immunohistochemistry: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753]

Immunohistochemistry: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753]

Immunohistochemistry: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753] - Staining of paraffin-embedded Human pancreas tissue using anti-DAP Kinase 2 mouse monoclonal antibody.
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Applications for DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunoprecipitation

2ug/500ul

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DAPK2

Protein kinase termed death-associated protein kinase 2 (DAPK2) dependant on calcium/calmodulin (Ca2+/CaM) contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a protein kinase involved in apoptosis. Overexpression of DAPK2 significantly induced the morphological changes characteristic of apoptosis. Results indicate that DAPK2 is an additional member of DAP kinase family involved in apoptotic signaling (1). The region of homology spans the catalytic domain and the CaM-regulatory region, whereas the remaining C-terminal part of the protein differs completely from DAP kinase and displays no homology to any known protein. The catalytic domain is also homologous to the recently identified ZIP kinase and to a lesser extent to the catalytic domains of DRAK1 and -2 (2). DAPK2 has a novel regulatory mechanism that controls its pro-apoptotic functions. It comprises a single autophosphorylation event mapped to Ser308 within the CaM regulatory domain. A negative charge at this site reduces both the binding to CaM and the formation of DRP-1 homodimers (3).

Long Name

Death-associated Protein Kinase 2

Alternate Names

DAP Kinase 2, DRP-1

Gene Symbol

DAPK2

Additional DAPK2 Products

Product Documents for DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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