DAP3 Antibody (42C617.1.2)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56539

Novus Biologicals, part of Bio-Techne
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NB100-56539
NB100-56539SS
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Azide Free
NBP2-33222
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 42C617.1.2

Concentration

0.5 mg/ml

View all DAP3 Antibodies »

Product Specifications

Immunogen

The antibody was raised against a synthetic peptide corresponding to amino acids 51-66 of human DAP-3 protein.

Reactivity Notes

75% sequence identity with rat protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Scientific Data Images for DAP3 Antibody (42C617.1.2)

Western Blot: DAP3 Antibody (42C617.1.2) [NB100-56539]

Western Blot: DAP3 Antibody (42C617.1.2) [NB100-56539]

Western Blot: DAP3 Antibody (42C617.1.2) [NB100-56539] - Analysis of DAP-3 in A) human brain, B) Jurkat and C) NIH 3T3 lysate using this antibody at 5 ug/ml.

Applications for DAP3 Antibody (42C617.1.2)

Application
Recommended Usage

Western Blot

3-5 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.2% gelatin

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: DAP3

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5). DAP-3 has an ATP/GTP binding motive (P-loop) and has been isolated from human and mouse, and named as hDAP-3 and mDAP-3 (Death Associated Protein-3), respectively. Sequence analysis revealed an 82% amino acid identity between human and mouse protein. In situ hybridization and northern blot analysis showed an abundant mRNA expression with a pronounced expression in highly proliferative epithelial compartments. Overexpression of DAP-3 leads to cytochrome c release and induction of cell death. DAP-3 mediated apoptosis was shown to depend on a functional P-loop. mDAP-3 is localized to the mitochondrial matrix and in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction.

Alternate Names

bMRP-10, DAP-3Death-associated protein 3, death associated protein 3, DKFZp686G12159,28S ribosomal protein S29, mitochondrial, FLJ12817, MGC126058, MGC126059, mitochondrial 28S ribosomal protein S29, MRP-S29Ionizing radiation resistance conferring protein, MRPS29S29mt

Entrez Gene IDs

7818 (Human); 65111 (Mouse)

Gene Symbol

DAP3

UniProt

P51398 (Human)

Additional DAP3 Products

Product Documents for DAP3 Antibody (42C617.1.2)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DAP3 Antibody (42C617.1.2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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