DBT Antibody (OTI1G2) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70553

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1G2

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all DBT Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DBT(NP_001909) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DBT Antibody (OTI1G2) - Azide and BSA Free

Western Blot: DBT Antibody (OTI1G2)Azide and BSA Free [NBP2-70553]

Western Blot: DBT Antibody (OTI1G2)Azide and BSA Free [NBP2-70553]

Western Blot: DBT Antibody (OTI1G2) - Azide and BSA Free [NBP2-70553] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DBT (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DBT.
Immunocytochemistry/ Immunofluorescence: DBT Antibody (OTI1G2) - Azide and BSA Free [NBP2-70553]

Immunocytochemistry/ Immunofluorescence: DBT Antibody (OTI1G2) - Azide and BSA Free [NBP2-70553]

Immunocytochemistry/Immunofluorescence: DBT Antibody (OTI1G2) - Azide and BSA Free [NBP2-70553] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DBT.
Flow Cytometry: DBT Antibody (OTI1G2) - Azide and BSA Free [NBP2-70553]

Flow Cytometry: DBT Antibody (OTI1G2) - Azide and BSA Free [NBP2-70553]

Flow Cytometry: DBT Antibody (OTI1G2) - Azide and BSA Free [NBP2-70553] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-DBT antibody, and then analyzed by flow cytometry.

Applications for DBT Antibody (OTI1G2) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: DBT

The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Alternate Names

BCATE2BCKAD E2 subunit, BCKADE2, BCKAD-E2, branched chain acyltransferase, E2 component, Branched-chain alpha-keto acid dehydrogenase complex component E2, Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto aciddehydrogenase complex, Dihydrolipoamide branched chain transacylase, dihydrolipoamide branched chain transacylase (E2 component of branched chainketo acid dehydrogenase complex; maple syrup urine disease), dihydrolipoamide branched chain transacylase E2, dihydrolipoyl transacylase, Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase, E2, E2 component of branched chain alpha-keto acid dehydrogenase complex, E2B, EC 2.3.1.168, lipoamide acyltransferase component of branched-chain alpha-keto aciddehydrogenase complex, mitochondrial, lipoamide acyltransferase component of mitochondrial branched-chain alpha-ketoacid dehydrogenase complex, MGC9061, mitochondrial branched chain alpha-keto acid dehydrogenase transacylase subunit(E2b)

Gene Symbol

DBT

Additional DBT Products

Product Documents for DBT Antibody (OTI1G2) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for DBT Antibody (OTI1G2) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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