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DDIT4L Antibody (DDIT-03) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62218CL1

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow (Intracellular), Flow Cytometry, Western Blot

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG1 Clone # DDIT-03

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

N-terminal recombinant fragment of human DDIT4L (amino acids 2-98)

Specificity

The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for DDIT4L Antibody (DDIT-03) [CoraFluor™ 1]

Application
Recommended Usage

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: DDIT4L

DDIT4L, also known as DNA damage-inducible transcript 4-like protein, is a 21.7 kDa 193 amino acid protein that regulates the TOR signaling pathway between the TSC1-TSC2 complex and AKT1, thus inhibiting cell growth. Current research is being conducted with diseases and disorders such as teratoma, melanoma, and hypoxia. The protein is involved in the TOR signaling pathway, where it interacts with SNRPG and PRKAB2.

Alternate Names

DNA damage-inducible transcript 4-like protein, DNA-damage-inducible transcript 4-like, HIF-1 responsive protein RTP801-like, homolog of mouse SMHS1, Protein regulated in development and DNA damage response 2, REDD-2, REDD2Rtp801L, regulated in development and DNA damage response 2, RTP801L

Gene Symbol

DDIT4L

Additional DDIT4L Products

Product Documents for DDIT4L Antibody (DDIT-03) [CoraFluor™ 1]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DDIT4L Antibody (DDIT-03) [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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