Dkk-1 Antibody (2A5)
Novus Biologicals, part of Bio-Techne | Catalog # H00022943-M11
Key Product Details
Species Reactivity
Validated:
Human, Yeast
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Microarray, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 2A5
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
DKK1 (AAH01539.1, 1 a.a. ~ 266 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MMALGAAGATRVSVAMVAAALGGHPLLGVSATLNSVLNSNAIKNLPPPLGGAAGHPGSAVSAAPGILYPGGNKYQTIDNYQPYPCAEDEECGTDEYCASPTRGGDAGVQICLACRKRRKRCMRHAMCCPGNYCKNGICVSSDQNHFRGEIEETITESFGNDHSTLDGYSRRTTLSSKMYHTTGQEGSVCLRSSDCASGLCCARHFWSKICKPVLKGGQVCTKHRRKGSHGLEIFQRCYCGEGLSCRIQKDHHQASNSSRLHTCQRH
Specificity
DKK1 - dickkopf homolog 1 (Xenopus laevis)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Scientific Data Images for Dkk-1 Antibody (2A5)
Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11]
Western Blot: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 expression in U-2 OS (Cat # L022V1).Immunohistochemistry-Paraffin: Dkk-1 Antibody (2A5) [H00022943-M11]
Immunohistochemistry-Paraffin: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of monoclonal antibody to DKK1 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/mlImmunoprecipitation: Dkk-1 Antibody (2A5) [H00022943-M11]
Immunoprecipitation: Dkk-1 Antibody (2A5) [H00022943-M11] - Analysis of DKK1 transfected lysate using anti-DKK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with DKK1 rabbit polyclonal antibody.Applications for Dkk-1 Antibody (2A5)
Application
Recommended Usage
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA. Immunocytochemistry/Immunofluorescence was reported in scientific literature.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Dkk-1
Long Name
Dickkopf-1
Alternate Names
Dkk1
Entrez Gene IDs
22943 (Human)
Gene Symbol
DKK1
OMIM
605189 (Human)
UniProt
Additional Dkk-1 Products
Product Documents for Dkk-1 Antibody (2A5)
Product Specific Notices for Dkk-1 Antibody (2A5)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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