Dkk-3 Antibody (103B5) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60242

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 103B5

Format

BSA Free

Concentration

1 mg/ml

View all Dkk-3 Antibodies »

Product Summary for Dkk-3 Antibody (103B5) - BSA Free

Immunogen

Recombinant human DKK-3 (22-350aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Dkk-3 Antibody (103B5) - BSA Free

Western Blot: Dkk-3 Antibody (103B5)BSA Free [NBP2-60242]

Western Blot: Dkk-3 Antibody (103B5)BSA Free [NBP2-60242]

Western Blot: Dkk-3 Antibody (103B5) [NBP2-60242] - The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Dkk3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: HepG2 cell lysate Lane 3.: MCF7 cell lysate Lane 4.: A549 cell lysate Lane 5.: Mouse brain tissue lysate Lane 6.: Mouse kidney tissue lysate

Applications for Dkk-3 Antibody (103B5) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Dkk-3

DKK3 is a secreted protein that is a member of the dickkopf family. It contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene.

Long Name

Dickkopf-3

Alternate Names

Dkk3, REIC

Gene Symbol

DKK3

Additional Dkk-3 Products

Product Documents for Dkk-3 Antibody (103B5) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Dkk-3 Antibody (103B5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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