Dkk-3 Antibody (1R5E0)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16293
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1R5E0
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 200-350 of human Dkk-3 (NP_056965.3). TRGSNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPASRLLDLITWELEPDGALDRCPCASGLLCQPHSHSLVYVCKPTFVGSRDQDGEILLPREVPDEYEVGSFMEEVRQELEDLERSLTEEMALREPAAAAAALLGGEEI
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Dkk-3 Antibody (1R5E0)
![Western Blot: Dkk-3 Antibody (1R5E0) [NBP3-16293]](https://resources.bio-techne.com/images/products/Dkk-3-Antibody-1R5E0-Western-Blot-NBP3-16293-img0002.jpg "Western Blot: Dkk-3 Antibody (1R5E0) [NBP3-16293]")
Western Blot: Dkk-3 Antibody (1R5E0) [NBP3-16293]
Western Blot: Dkk-3 Antibody (1R5E0) [NBP3-16293] - Western blot analysis of extracts of various cell lines, using Dkk-3 Rabbit mAb (NBP3-16293) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.![Immunocytochemistry/ Immunofluorescence: Dkk-3 Antibody (1R5E0) [NBP3-16293]](https://resources.bio-techne.com/images/products/Dkk-3-Antibody-1R5E0-Immunocytochemistry-Immunofluorescence-NBP3-16293-img0001.jpg "Immunocytochemistry/ Immunofluorescence: Dkk-3 Antibody (1R5E0) [NBP3-16293]")
Immunocytochemistry/ Immunofluorescence: Dkk-3 Antibody (1R5E0) [NBP3-16293]
Immunocytochemistry/Immunofluorescence: Dkk-3 Antibody (1R5E0) [NBP3-16293] - Immunofluorescence analysis of SH-SY5Y cells using Dkk-3 Rabbit mAb (NBP3-16293) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for Dkk-3 Antibody (1R5E0)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Dkk-3
Long Name
Dickkopf-3
Alternate Names
Dkk3, REIC
Gene Symbol
DKK3
Additional Dkk-3 Products
Product Documents for Dkk-3 Antibody (1R5E0)
Product Specific Notices for Dkk-3 Antibody (1R5E0)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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