DMPK Antibody (2F7)

Novus Biologicals, part of Bio-Techne | Catalog # H00001760-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F7

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DMPK Antibodies »

Product Specifications

Immunogen

DMPK (AAH62553, 303 a.a. ~ 420 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DEGVPEEARDFIQRLLCPPETRLGRGGAGDFRTHPFFFGLDWDGLRDSVPPFTPDFEGATDTCNFDLVEDGLTAMVSGGGETLSDIREGAPLGVHLPFVGYSYSCMALRDSEVPGPTP

Specificity

DMPK - dystrophia myotonica-protein kinase

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for DMPK Antibody (2F7)

Western Blot: DMPK Antibody (2F7) [H00001760-M01]

Western Blot: DMPK Antibody (2F7) [H00001760-M01]

Western Blot: DMPK Antibody (2F7) [H00001760-M01] - Analysis of DMPK expression in transfected 293T cell line by DMPK monoclonal antibody (M01), clone 2F7.Lane 1: DMPK transfected lysate(69.4 KDa).Lane 2: Non-transfected lysate.
Western Blot: DMPK Antibody (2F7) [H00001760-M01]

Western Blot: DMPK Antibody (2F7) [H00001760-M01]

Western Blot: DMPK Antibody (2F7) [H00001760-M01] - Analysis of DMPK over-expressed 293 cell line, cotransfected with DMPK Validated Chimera RNAi ( Cat # H00001760-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with DMPK monoclonal antibody (M01) clone 2F7 (Cat # H00001760-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
ELISA: DMPK Antibody (2F7) [H00001760-M01]

ELISA: DMPK Antibody (2F7) [H00001760-M01]

ELISA: DMPK Antibody (2F7) [H00001760-M01] - Detection limit for recombinant GST tagged DMPK is approximately 0.03ng/ml as a capture antibody.

Applications for DMPK Antibody (2F7)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DMPK

The protein encoded by this gene is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-37 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5,000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Long Name

Dystrophia Myotonica Protein Kinase

Alternate Names

DM1PK, DMK, MDPK, MT-PK

Entrez Gene IDs

1760 (Human)

Gene Symbol

DMPK

OMIM

160900 (Human)

UniProt

AAH62553 (Human)

Additional DMPK Products

Product Documents for DMPK Antibody (2F7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for DMPK Antibody (2F7)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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