DNMT1 Antibody (1K5K8)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15856
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
ELISA, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1K5K8
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for DNMT1 Antibody (1K5K8)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNMT1 (NP_001370.1). MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYN
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
183 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for DNMT1 Antibody (1K5K8)
![Immunoprecipitation: DNMT1 Antibody (1K5K8) [NBP3-15856]](https://resources.bio-techne.com/images/products/DNMT1-Antibody-8E3H1-Immunoprecipitation-NBP3-15856-img0008.jpg "Immunoprecipitation: DNMT1 Antibody (1K5K8) [NBP3-15856]")
Immunoprecipitation: DNMT1 Antibody (1K5K8) [NBP3-15856]
Immunoprecipitation: DNMT1 Antibody (8E3H1) [NBP3-15856] - Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug DNMT1 antibody (NBP3-15856). Western blot was performed from the immunoprecipitate using DNMT1 (NBP3-15856) at a dilition of 1:1000.
Western Blot: DNMT1 Antibody (8E3H1) [NBP3-15856] -
Western Blot: DNMT1 Antibody (8E3H1) [NBP3-15856] - Analysis of lysates from wild type (WT) and DNMT1 knockdown (KD) 293T cells, using [KD Validated] DNMT1 Rabbit mAb at1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.
Western Blot: DNMT1 Antibody (8E3H1) [NBP3-15856] -
Western Blot: DNMT1 Antibody (8E3H1) [NBP3-15856] - Analysis of various lysates using [KD Validated] DNMT1 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30 s.Applications for DNMT1 Antibody (1K5K8)
Application
Recommended Usage
Immunoprecipitation
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: DNMT1
Improper methylation patterns have been linked to certain human tumors and developmental abnormalities. DNMT1 antibodies are useful tools for DNA methlyation research and studies on certian cancer.
Long Name
DNA [Cytosine-5-]-Methyltransferase 1
Alternate Names
CXXC9, MCMT
Gene Symbol
DNMT1
Additional DNMT1 Products
Product Documents for DNMT1 Antibody (1K5K8)
Product Specific Notices for DNMT1 Antibody (1K5K8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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