DOT1L Antibody (3B6) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15150
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3B6
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human DOT1L. UniProt ID (Q8TEK3).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for DOT1L Antibody (3B6) - BSA Free
Western Blot: DOT1L Antibody (3B6) [NBP3-15150]
Western Blot: DOT1L Antibody (3B6) [NBP3-15150] - Positive WB detected in: A549 whole cell lysate, Hela whole cell lysate, 293T whole cell lysateAll lanes: DOT1L antibody at 1:1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 165 kDa
Observed band size: 165 kDa
Immunocytochemistry/Immunofluorescence: DOT1L Antibody (3B6) [NBP3-15150]
Immunocytochemistry/Immunofluorescence: DOT1L Antibody (3B6) [NBP3-15150] - Staining of Hela cell with NBP3-15150 at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).Applications for DOT1L Antibody (3B6) - BSA Free
Application
Recommended Usage
Western Blot
1:500-1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl, and 50% glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: DOT1L
Long Name
Disruptor of Telomeric Silencing 1-like
Alternate Names
H3-K79-HMTase, KMT4
Gene Symbol
DOT1L
Additional DOT1L Products
Product Documents for DOT1L Antibody (3B6) - BSA Free
Product Specific Notices for DOT1L Antibody (3B6) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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