DOT1L Antibody (PSH01-57)

Western Blot: DOT1L Antibody (PSH01-57) [NBP3-32278]

Western Blot: DOT1L Antibody (PSH01-57) [NBP3-32278] - Western blot analysis of DOT1L on different lysates with Rabbit anti-DOT1L antibody (NBP3-32278) at 1/1,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: THP-1 cell lysate
Lane 3: HeLa cell lysate
Lane 4: 293T cell lysate

Lysates/proteins at 40 ug/Lane.

Predicted band size: 165 kDa
Observed band size: 180 kDa

Exposure time: 1 minute 40 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32278) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: DOT1L Antibody (PSH01-57) [NBP3-32278]

Immunohistochemistry: DOT1L Antibody (PSH01-57) [NBP3-32278] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-DOT1L antibody (NBP3-32278) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32278) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.