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DOT1L Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-40846

Novus Biologicals, part of Bio-Techne
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NB100-40846

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue1485 and the C-terminus (residue 1537) of human DOT1-Like using the numbering given in entry NP_115871.1 (GeneID 84444).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for DOT1L Antibody

Immunoprecipitation: DOT1L Antibody [NB100-40846]

Immunoprecipitation: DOT1L Antibody [NB100-40846]

Immunoprecipitation: DOT1L Antibody [NB100-40846] - Detection of Human DOT1L by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-DOT1L antibody NB100-40846 used for IP at 3 mcg/mg lysate. DOT1L was also immunoprecipitated by rabbit anti- DOT1L antibody NB100-40845, which recognizes an upstream epitope. Detection: Chemiluminescence with an exposure time 10 seconds.

Applications for DOT1L Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: DOT1L

DOT1 is an evolutionarily conserved histone methyltransferase that methylates lysine-79 of histone H3 in the core domain. Unlike other histone methyltransferases, DOT1 does not contain a SET domain, and it specifically methylates nucleosomal histone H3.

Long Name

Disruptor of Telomeric Silencing 1-like

Alternate Names

H3-K79-HMTase, KMT4

Entrez Gene IDs

84444 (Human)

Gene Symbol

DOT1L

Additional DOT1L Products

Product Documents for DOT1L Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DOT1L Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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