DPPIV/CD26 Antibody (202.36) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47256
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Rat, Porcine (Negative), Sheep (Negative)
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 202.36
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Human T cell clone
Reactivity Notes
Does not react with Porcine or Sheep.
Localization
Cell Surface
Specificity
Recognizes a glycoprotein of 110kDa, identified as CD26 (Workshop VI; Code: N-L039). It is an atypical serine protease belonging to the prolyl oligopeptidase family. It is expressed on lymphocyte cells and is upregulated during T-cell activation. CD26 is also expressed on activated B cells and natural killer cells and abundantly on epithelia. CD26 is implicated in a variety of biological functions including T-cell activation, cell adhesion with extracellular matrix such as fibronectin or collagens, and in HIV infection. Cross-linking of CD26 using this antibody dramatically enhances the anti-CD3-induced IL-2 production. In Western blotting, this monoclonal antibody reacts with only glycosylated CD26, but not with the deglycosylated form. It does not prevent ADA binding to CD26.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
110 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44571).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Applications for DPPIV/CD26 Antibody (202.36) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Frozen
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Frozen Only): 1-2ug/ml for 30 minutes at RT.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: DPPIV/CD26
Long Name
Dipeptidyl-peptidase IV
Alternate Names
CD26, DPP4
Gene Symbol
DPP4
Additional DPPIV/CD26 Products
Product Documents for DPPIV/CD26 Antibody (202.36) - Azide and BSA Free
Product Specific Notices for DPPIV/CD26 Antibody (202.36) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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