DUOX1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16232

Novus Biologicals, part of Bio-Techne
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NBP2-16232
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Bovine (90%), Porcine (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all DUOX1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human DUOX1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Canine (86%), Rat (87%).

Localization

Apical cell membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

177 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for DUOX1 Antibody

Western Blot: DUOX1 Antibody [NBP2-16232]

Western Blot: DUOX1 Antibody [NBP2-16232]

Western Blot: DUOX1 Antibody [NBP2-16232] - Boiled and unboiled A431 whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DUOX1 antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract).
Immunohistochemistry-Paraffin: DUOX1 Antibody [NBP2-16232]

Immunohistochemistry-Paraffin: DUOX1 Antibody [NBP2-16232]

Immunohistochemistry-Paraffin: DUOX1 Antibody [NBP2-16232] - Human gastric cancer, using DUOX1 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Western Blot: DUOX1 Antibody [NBP2-16232]

Western Blot: DUOX1 Antibody [NBP2-16232]

Western Blot: DUOX1 Antibody [NBP2-16232] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DUOX1 antibody [N1N3] diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Applications for DUOX1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: DUOX1

The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX2. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq]

Alternate Names

dual oxidase 1, DUOX, EC 1.11.1.-, EC 1.6.3.1, Large NOX 1, LNOX1flavoprotein NADPH oxidase, Long NOX 1, MGC138840, NADPH thyroid oxidase 1, nicotinamide adenine dinucleotide phosphate oxidase, NOXEF1, THOX1MGC138841, Thyroid oxidase 1

Gene Symbol

DUOX1

UniProt

Q9NRD9 (Human)

Additional DUOX1 Products

Product Documents for DUOX1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for DUOX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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