E-Cadherin Antibody (056)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89338
Recombinant Monoclonal Antibody
![Western Blot: E-Cadherin Antibody (056) [NBP2-89338]](https://resources.bio-techne.com/images/products/E-Cadherin-Antibody-056-Western-Blot-NBP2-89338-img0002.jpg "Western Blot: E-Cadherin Antibody (056) [NBP2-89338]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Porcine
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 056
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was obtained from a rabbit immunized with purified, recombinant Human E-Cadherin (Accession#: NP_004351.1; Met1-Ile707).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for E-Cadherin Antibody (056)
Western Blot: E-Cadherin Antibody (056) [NBP2-89338]
Western Blot: E-Cadherin Antibody (056) [NBP2-89338] - Lane A: MCF7 Whole Cell Lysate Lane B: A431 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:97 kDa Observed band size:130 kDa![Immunoprecipitation: E-Cadherin Antibody (056) [NBP2-89338]](https://resources.bio-techne.com/images/products/E-Cadherin-Antibody-056-Immunoprecipitation-NBP2-89338-img0001.jpg "Immunoprecipitation: E-Cadherin Antibody (056) [NBP2-89338]")
Immunoprecipitation: E-Cadherin Antibody (056) [NBP2-89338]
Immunoprecipitation: E-Cadherin Antibody (056) [NBP2-89338] - Lane A:0.5 mg MCF-7 Whole Cell Lysate Lane B:0.5 mg A431 Whole Cell Lysate4 uL anti-E-cad rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody: Anti-E-cad rabbit monoclonal antibody,at 1:100 dilution Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 97 kDa Observed band size: 97 kDaApplications for E-Cadherin Antibody (056)
Application
Recommended Usage
Immunoprecipitation
4-8 uL/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: E-Cadherin
Alternate Names
Arc-1, CAD1, Cadherin-1, CD324, CDH1, Cell-CAM120/80, ECAD, ECadherin, L-CAM, Uvomorulin
Gene Symbol
CDH1
Additional E-Cadherin Products
Product Documents for E-Cadherin Antibody (056)
Product Specific Notices for E-Cadherin Antibody (056)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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