Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3A9

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Endoglin/CD105 Antibodies »

Product Summary for Endoglin/CD105 Antibody (3A9) - Azide and BSA Free

Immunogen

Purified recombinant fragment of human CD105 expressed in E. coli.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 27322553).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

71 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Endoglin/CD105 Antibody (3A9) - Azide and BSA Free

Western Blot: Endoglin/CD105 Antibody (3A9)Azide and BSA Free [NBP2-80719]

Western Blot: Endoglin/CD105 Antibody (3A9)Azide and BSA Free [NBP2-80719]

Western Blot: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Whole cell protein from HUVEC was separated on a 7.5% gel polyacrylamide gel and transferred to PVDF membrane. The membrane was probed with anti-CD105 antibody at 2 ug/ml and detected with an anti-mouse HRP labeled secondary antibody using chemiluminescence. Image from the standard format of this antibody.
Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719]

Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719]

Immunocytochemistry/Immunofluorescence: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - HMSC cells stained with anti-CD105 antibody. Image from verified customer review. Image from the standard format of this antibody.
Immunohistochemistry: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719]

Immunohistochemistry: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719]

Immunohistochemistry: Endoglin/CD105 Antibody (3A9) - Azide and BSA Free [NBP2-80719] - Analysis of paraffin-embedded kidney cancer tissues (left) and stomach cancer tissues (right) using CD105 mouse mAb with DAB staining. Image from the standard format of this antibody.
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Applications for Endoglin/CD105 Antibody (3A9) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:10000

Immunocytochemistry/ Immunofluorescence

1:200-1:1000. Use reported by customer review

Immunohistochemistry-Paraffin

1:200-1:1000

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Endoglin/CD105

This gene encodes a homodimeric transmembrane protein which is a major glycoprotein of the vascular endothelium. This protein is a component of the transforming growth factor beta receptor complex and it binds TGFB1 and TGFB3 with high affinity. Mutations in this gene cause hereditary hemorrhagic telangiectasia, also known as Osler-Rendu-Weber syndrome 1, an autosomal dominant multisystemic vascular dysplasia.

Alternate Names

CD105, ENG

Gene Symbol

ENG

Additional Endoglin/CD105 Products

Product Documents for Endoglin/CD105 Antibody (3A9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Endoglin/CD105 Antibody (3A9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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