Endoglin/CD105 Antibody (MEM-226) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB500-452

Novus Biologicals, part of Bio-Techne
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NB500-452
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Rat

Applications

Validated:

CyTOF-ready, Flow (Cell Surface), Flow Cytometry, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # MEM-226

Format

BSA Free

Concentration

1 mg/ml

View all Endoglin/CD105 Antibodies »

Product Specifications

Immunogen

Recombinant full length protein (Human). Expressed in vaccinia virus containing CD105 cDNA.

Localization

Cell Membrane

Specificity

The antibody MEM-226 reacts with CD105 (Endoglin), a 90 kDa type I homodimerizing membrane glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.

Marker

Neo-endothelial Cells Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Endoglin/CD105 Antibody (MEM-226) - BSA Free

Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) - BSA Free [NB500-452]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) - BSA Free [NB500-452]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - A cell surface stain was performed on HeLa cells with NB500-452F (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to FITC.
Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) - BSA Free [NB500-452]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) - BSA Free [NB500-452]

Flow Cytometry: Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Surface staining (mass cytometry) of hTERT cell line with anti-human CD105 (MEM-226) 143Nd. Gated on singlets.
Flow (Cell Surface): Endoglin/CD105 Antibody (MEM-226) - BSA Free [NB500-452]

Flow (Cell Surface): Endoglin/CD105 Antibody (MEM-226) - BSA Free [NB500-452]

Flow (Cell Surface): Endoglin/CD105 Antibody (MEM-226) [NB500-452] - Analysis using the FITC conjugate of NB500-452. Staining of HUVEC (human umbilical vein endothelial cells) with anti-human CD105 (MEM-226) FITC. Total viable cells were used for analysis.
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Applications for Endoglin/CD105 Antibody (MEM-226) - BSA Free

Application
Recommended Usage

Flow Cytometry

1-5 ug/ml

Immunoprecipitation

1:50

Western Blot

1:1000
Application Notes
Western Blot validation of this product was carried out under non-reducing conditions. This antibody is CyTOF ready.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.9% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Endoglin/CD105

CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbetaR-2 as a receptor for TGFbeta-1 and TGFbeta-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFbeta-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.

Alternate Names

CD105, ENG

Gene Symbol

ENG

Additional Endoglin/CD105 Products

Product Documents for Endoglin/CD105 Antibody (MEM-226) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Endoglin/CD105 Antibody (MEM-226) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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