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Endoglin/CD105 Antibody (MEM-229) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-81749

Novus Biologicals, part of Bio-Techne
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NB110-81749
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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Canine (Negative), Equine (Negative)

Cited:

Porcine

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Flow Cytometry

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # MEM-229

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

recombinant vaccinia virus containing human CD105 (L-isoform) cDNA

Specificity

This antibody (clone MEM-229) recognizes CD105 (Endoglin), a 180 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.

Marker

Neo-endothelial Cells Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Applications for Endoglin/CD105 Antibody (MEM-229) [FITC]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Size Exclusion Chromatography

Formulation

Stabilizing phosphate buffered saline (PBS).

Preservative

0.9% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Endoglin/CD105

CD105 (Endoglin) is a homodimeric transmembrane glycoprotein serving in presence of TGFbR-2 as a receptor for TGFb-1 and TGFb-3. CD105 is highly expressed on endothelial cells and promotes angiogenesis during wound healing, infarcts and in a wide range of tumours and its gene expression is stimulated by hypoxia. CD105 prevents apoptosis in hypoxic endothelial cells and also antagonises the inhibitory effects of TGFb-1 on vascular endothelial cell growth and migration. Normal cellular levels of CD105 are required for formation of new blood vessels.

Alternate Names

CD105, ENG

Gene Symbol

ENG

Additional Endoglin/CD105 Products

Product Documents for Endoglin/CD105 Antibody (MEM-229) [FITC]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Endoglin/CD105 Antibody (MEM-229) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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