Endoglin/CD105 Antibody (MEM-229) [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NB110-81749
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Porcine, Canine (Negative), Equine (Negative)
Cited:
Porcine
Applications
Validated:
Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Cited:
Flow Cytometry
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # MEM-229
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
recombinant vaccinia virus containing human CD105 (L-isoform) cDNA
Specificity
This antibody (clone MEM-229) recognizes CD105 (Endoglin), a 180 kDa type I integral membrane homodimer glycoprotein expressed on vascular endothelial cells (small and large vessels), activated monocytes and tissue macrophages, stromal cells of certain tissues including bone marrow, pre-B lymphocytes in fetal marrow and erythroid precursors in fetal and adult bone marrow; it is also present on syncytiotrophoblast on placenta throughout pregnancy.
Marker
Neo-endothelial Cells Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Theoretical MW
90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Applications for Endoglin/CD105 Antibody (MEM-229) [FITC]
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.
Formulation, Preparation, and Storage
Purification
Size Exclusion Chromatography
Formulation
Stabilizing phosphate buffered saline (PBS).
Preservative
0.9% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Endoglin/CD105
Alternate Names
CD105, ENG
Gene Symbol
ENG
Additional Endoglin/CD105 Products
Product Documents for Endoglin/CD105 Antibody (MEM-229) [FITC]
Product Specific Notices for Endoglin/CD105 Antibody (MEM-229) [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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