Endosialin/CD248/TEM1 Antibody [CoraFluor™ 1]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77311CL1
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Mouse (100%), Rat (93%). Backed by our 100% Guarantee.
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
CoraFluor 1
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human TEM-1. The immunogen is located within amino acids 40-90 of TEM-1 Amino Acid Squence: ATPRTPEEAQRVDS
Specificity
Human TEM-1 has 2 isoforms, including isoform 1 (757aa, 81kD), and isoform 2 (433aa, 46kD). Mouse TEM-1 has one isoform (765aa, 82kD) and Rat TEM-1 also has one isoform (764aa, 82kD). NBP1-77311 can detect human isoform 1, mouse and rat TEM-1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Applications for Endosialin/CD248/TEM1 Antibody [CoraFluor™ 1]
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark. Do not freeze.
Background: Endosialin/CD248
Alternate Names
CD164L1, CD248, TEM1
Gene Symbol
CD248
Additional Endosialin/CD248 Products
Product Documents for Endosialin/CD248/TEM1 Antibody [CoraFluor™ 1]
Product Specific Notices for Endosialin/CD248/TEM1 Antibody [CoraFluor™ 1]
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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