Endosialin/CD248/TEM1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14462
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Cited:
Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against a recombinant protein corresponding to the amino acids: EEDEDEAWKAFNGGWTEMPGILWMEPTQPPDFALAYRPSFPEDREPQIPYPEPTWPPPLSAPRVPYHSSVLSVTRPVVVSATH
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 26300407).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Endosialin/CD248/TEM1 Antibody
Immunocytochemistry/ Immunofluorescence: Endosialin/CD248/TEM1 Antibody [NBP2-14462]
Immunocytochemistry/Immunofluorescence: Endosialin/CD248/TEM1 Antibody [NBP2-14462] - Staining of human cell line ASC TERT1 shows localization to nucleoplasm & plasma membrane. Antibody staining is shown in green.Immunohistochemistry-Paraffin: Endosialin/CD248/TEM1 Antibody [NBP2-14462]
Immunohistochemistry-Paraffin: Endosialin/CD248/TEM1 Antibody [NBP2-14462] - Staining of human placenta shows high expression.Applications for Endosialin/CD248/TEM1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Endosialin/CD248
Alternate Names
CD164L1, CD248, TEM1
Gene Symbol
CD248
Additional Endosialin/CD248 Products
Product Documents for Endosialin/CD248/TEM1 Antibody
Product Specific Notices for Endosialin/CD248/TEM1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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